Human PPARG Gene Mutation as Risk Factor for Type 2 Diabetes Melitus: In Silico Analysis

Authors

  • Naufal Yafi Rais Wiguna
  • Taufik Hidayat B
  • Putrya Hawa
  • Jonny

Keywords:

Type 2 Diabetes Mellitus, PPARG, Bioinformatics, In Silico, SNPs

Abstract

Introduction: Type 2 diabetes (T2DM) involves genetic and environmental factors. PPARG, encoding peroxisome proliferator-activated receptor gamma, is one of the key gene in T2DM development. Our study investigates PPARG variants' role as risk factor for T2DM by in silico analysis.

Methods: We identified Single Nucleotide Polymorphisms (SNPs) within the PPARG gene via UniProt and analyzed their effects using Ensembl Variant Effect Predictor (VEP). The VEP analysis provided us four important indicators: impact assessment, Sorting Intolerant from Tolerant (SIFT) score, PolyPhen score, and clinical significance. We also investigated PPARG's interactions with other T2DM-related genes using StringDB.

Results:  In our analysis of 35 UniProt-sourced SNPs, 32 underwent successful VEP analysis.The  SIFT indicators identified 23 of SNPs  as deleterious, The PolyPhen identified 18 of SNPs  as probably damaging . Impact assessments revealed that 27 had a moderate impact  on gene function. Clinically, 8 of the SNPs were considered pathogenic and rs1805192 emerged as a notable risk factor for T2DM. Additionally, StringDB analysis confirmed PPARG's role in the T2DM-associated gene network, from the 25 proteins involved in T2DM, 21 of them exhibit correlations with PPARG.

Discussion: PPARG SNPs variant has a significant impact on T2DM as a risk factor. However, SNPs associated with T2DM vary across different populations.

Conclusion:  Analysis of PPARG genetic variations highlights their significant association with T2DM susceptibility in specific populations. Bioinformatics tools are useful for investigating genetic mutations but require additional research, such as functional studies, to improve reliability as their outcomes are primarily predictions.

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Author Biographies

Naufal Yafi Rais Wiguna

Faculty of Military Medicine, Republic of Indonesia Defense University, Bogor, Indonesia

Taufik Hidayat B

Faculty of Military Medicine, Republic of Indonesia Defense University, Bogor, Indonesia

Putrya Hawa

Faculty of Military Medicine, Republic of Indonesia Defense University, Bogor, Indonesia

Jonny

Faculty of Military Medicine, Republic of Indonesia Defense University, Bogor, Indonesia

Faculty of Medicine, Jakarta “Veterant” National Development University, Jakarta, Indonesia

Department of Internal Medicine, Gatot Soebroto Central Army Hospital, Jakarta, Indonesia

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Published

2024-01-03

How to Cite

Yafi Rais Wiguna, N. ., Hidayat B, T. ., Hawa, P. ., & Jonny, J. (2024). Human PPARG Gene Mutation as Risk Factor for Type 2 Diabetes Melitus: In Silico Analysis. ASEAN Journal of Military and Preventive Medicine, 1(1), 1–12. Retrieved from http://journal.ajmpm.net/index.php/ojs/article/view/1

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